Inflammation is a complex process that involves various signaling pathways. While individual cytokine levels like IL-6, TNF, and IL-1β can offer some insight into overall inflammatory status, they are not definitive markers. Chronic inflammation is linked to age-related diseases, but the correlation is not strong. Elevated levels of these cytokines are associated with diseases, disability, and mortality in older adults. Research suggests that monitoring pro-inflammatory cytokines in elderly individuals with comorbidities could improve treatment outcomes. To explore this further, a systematic review and meta-analysis focused on IL-6, TNF, and IL-1β levels in elderly individuals with comorbidities compared to disease-free controls. The results indicated higher IL-6 levels in patients with diseases, while TNF and IL-1β levels were elevated in controls. Further research is needed to understand the relationship between inflammatory markers and age-related diseases.